The main aim of Chromatrap® is to assist with the development of epigenetic research using its bead-free system. It involves the use of a Chromatrap filtration plate.
According to Chromatrap®, the patented filter used in Chromatrap® was first noticed by academics at Essex University and then later developed and collaborated with Swansea University’s Medical School.
The journey began with a kit designed for PCR analysis, and then the partnership inspired the development of a diverse product range offering a faster, easier and more sensitive method of chromatin immunoprecipitation.
The Chromatrap filtration increases the surface area, in turn, maximising immunocapture of antibody-chromatin complexes from your samples.
It is nothing like other bead-based kits suppliers such as Active Motif, Diagenode, Cell Signalling Technology, Abcam, Zymo Research and Epigentik. Chromatrap is a novel filter-based system that overcomes the challenges often encountered through the use of beads, in particular for first-time users.
All Chromatrap® products are manufactured at the Porvair Sciences biosciences facilities in North Wales and now sold worldwide.
If you would like to know how the Chromatrap® technology works, you can go online and visit their technology section. There are specific methods and descriptions along with diagrams.
The following is a list of what Chromatrap® has to offer:
- It is easy to use containing asimple spin-column protocol with no fiddly wash steps
- It has an unrivalled low background, theinert filter surface repels non-specific binding
- It has a fast workflow, producinghigh-quality data in under 5 hours
- There is no sample loss – the sample is captured and retained on the filter until elution
- There is less manual handling which means less handling errors
- High throughput capability process 96 samples in parallel on a single plate
According to Chromatrap®, it is best for sensitivity and creates correctly orientated proteins which ensure binding pockets are presented to the antibody-chromatin complex.
It also has an exceptional signal-to-noise inert filtration material coupled with superior orientation technology which ensures you enrich more of the chromatin you want without capturing the protein you don’t.
Furthermore, a high binding capacity of optimally orientated highly ordered protein arrangement throughout the filter provides a maximised surface area for binding.
If you want to know how Chromatrap® works in the laboratory, Chromatrap® and the team have created statistics between Chromatrap® vs competitors and Chromatrap® vs beads.
Compared to its competitors, Chromatrap® has up to 10x higher signal, and requires the lowest amount input material. It also produces thehighest DNA yield, has no non-specific binding and is the Best signal-to-noise of any ChIP kit.
On the other hand, compared to beads, Chromatrap® has the highest positive target enrichment, lowest negative target pull-down, the broadest dynamic range 50ng – 50µg, a shorter protocol, more straightforward process, and also no sample loss.
Chromatrap® is undoubtedly here to shake up the world of chromatin immunoprecipitation (ChIP). The dedicated technical team continues to improve and innovate the technology and its protocols to ensure that researchers get the results they want, allowing them to focus on the more significant questions in epigenetics research.
Contact the team at Chromatrap® if you have any questions or queries about Chromatrap filtration. You can fill in their form online at https://www.chromatrap.com/, and one of their team members will be in touch.
Chromatrap® is here to shake up the world of chromatin immunoprecipitation (ChIP) by offering researchers a new way of performing ChIP, effortlessly. Our goal is to enable the advancement of epigenetic research through our revolutionary bead-free system (that’s right, no beads).
Contact ChromatrapClywedog Rd S
Wrexham Industrial Estate
+44 (0)1978 661144